Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 350816 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#350816, RRID:AB_2563226
- Product name
- Alexa Fluor® 594 anti-human CD325 (N-Cadherin)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human N-cadherin extracellular domain
- Description
- CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 8C11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human acute myeloid leukemia cell line (KG1) cells were fixed with 2% paraformaldehyde (PFA) and blocked for 30 minutes with 5% FBS. Then the cells were stained with 5 µg/ml of Alexa Fluor® 594 anti-human CD325 (N-Cadherin) (clone 8C11) (red) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
- Conjugate
- Yellow dye