Antibody data
- Antibody Data
- Antigen structure
- References [41]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PA1328 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-N-Cadherin-2 CDH2 CD325-Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for N CADHERIN/CDH2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, FCM. Reactive species: Human;Mouse;Rat. N CADHERIN/CDH2 information: Molecular Weight: 99809 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer.
PD-L1 knockdown suppresses vasculogenic mimicry of non-small cell lung cancer by modulating ZEB1-triggered EMT.
PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3.
SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.
SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells.
Microtubule-associated protein 4 promotes epithelial mesenchymal transition in hepatocellular cancer cells via regulating GSK3β/β-catenin pathway.
CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling.
Downregulation of MAL2 inhibits breast cancer progression through regulating β-catenin/c-Myc axis.
Discovery of a Novel Bloom's Syndrome Protein (BLM) Inhibitor Suppressing Growth and Metastasis of Prostate Cancer.
DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression.
Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer.
Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter.
Lycorine upregulates the expression of RMB10, promotes apoptosis and inhibits the proliferation and migration of cervical cancer cells.
LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer.
Isoflurane promotes epithelial-to-mesenchymal transition and metastasis of bladder cancer cells through HIF-1α-β-catenin/Notch1 pathways.
Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.
Hsa_circ_001569 is an unfavorable prognostic factor and promotes cell proliferation and metastasis by modulating PI3K-AKT pathway in breast cancer.
Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1.
Interleukin-27 Inhibits Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition in Preeclampsia.
Immunomodulatory effect of human amniotic epithelial cells on restoration of ovarian function in mice with autoimmune ovarian disease.
Up-regulated circular RNA hsa_circ_0067934 contributes to glioblastoma progression through activating PI3K-AKT pathway.
Hsa_circ_0005075 predicts a poor prognosis and acts as an oncogene in colorectal cancer via activating Wnt/β-catenin pathway.
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells.
MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7.
Anti-cancer effect of Aquaporin 5 silencing in colorectal cancer cells in association with inhibition of Wnt/β-catenin pathway.
Andrographolide Induces Autophagic Cell Death and Inhibits Invasion and Metastasis of Human Osteosarcoma Cells in An Autophagy-Dependent Manner.
E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin.
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma.
ANXA1 affects cell proliferation, invasion and epithelial-mesenchymal transition of oral squamous cell carcinoma.
MgCl(2) and ZnCl(2) promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway.
Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage.
Different miR-21-3p isoforms and their different features in colorectal cancer.
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition.
SOX9 Overexpression Promotes Glioma Metastasis via Wnt/β-Catenin Signaling.
Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer.
Expression of N-cadherin in myocardial tissues during the development of a rat heart.
Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/β-catenin pathway in laryngeal cancer Hep-2 cells.
Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin.
Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-beta1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 expressions.
Wu J, Li W, Zhang X, Shi F, Jia Q, Wang Y, Shi Y, Wu S, Wang X
Scientific reports 2024 May 28;14(1):12228
Scientific reports 2024 May 28;14(1):12228
PD-L1 knockdown suppresses vasculogenic mimicry of non-small cell lung cancer by modulating ZEB1-triggered EMT.
Li W, Wu J, Jia Q, Shi Y, Li F, Zhang L, Shi F, Wang X, Wu S
BMC cancer 2024 May 23;24(1):633
BMC cancer 2024 May 23;24(1):633
PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3.
Gong H, Yang X, An L, Zhang W, Liu X, Shu L, Yang L
Molecular and cellular biochemistry 2024 Mar 29;
Molecular and cellular biochemistry 2024 Mar 29;
SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.
Chen S, Zhou Y, Peng P, Xu L, Tang Q, Chen W, Gu W
International journal of molecular sciences 2023 Oct 26;24(21)
International journal of molecular sciences 2023 Oct 26;24(21)
SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells.
Kang K, Ma YD, Liu SQ, Huang RW, Chen JJ, An LL, Wu J
Viruses 2023 May 29;15(6)
Viruses 2023 May 29;15(6)
Microtubule-associated protein 4 promotes epithelial mesenchymal transition in hepatocellular cancer cells via regulating GSK3β/β-catenin pathway.
Hu P, Zong B, Chen Q, Shao R, Chen M, Yang Y, Shao G
Heliyon 2023 Mar;9(3):e14309
Heliyon 2023 Mar;9(3):e14309
CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling.
Lei S, Du X, Tan K, He X, Zhu Y, Zhao S, Yang Z, Dou G
Experimental and therapeutic medicine 2023 Jul;26(1):314
Experimental and therapeutic medicine 2023 Jul;26(1):314
Downregulation of MAL2 inhibits breast cancer progression through regulating β-catenin/c-Myc axis.
An L, Gong H, Yu X, Zhang W, Liu X, Yang X, Shu L, Liu J, Yang L
Cancer cell international 2023 Jul 21;23(1):144
Cancer cell international 2023 Jul 21;23(1):144
Discovery of a Novel Bloom's Syndrome Protein (BLM) Inhibitor Suppressing Growth and Metastasis of Prostate Cancer.
Ma XY, Xu HQ, Zhao JF, Ruan Y, Chen B
International journal of molecular sciences 2022 Nov 26;23(23)
International journal of molecular sciences 2022 Nov 26;23(23)
DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression.
Dong C, Huang S, Sun L, Yao J, Yan J, Yin X
Scientific reports 2022 Nov 17;12(1):19775
Scientific reports 2022 Nov 17;12(1):19775
Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer.
Bai X, Liu X, Li S, An H, Kang X, Guo S
Journal of agricultural and food chemistry 2022 Mar 30;70(12):3687-3696
Journal of agricultural and food chemistry 2022 Mar 30;70(12):3687-3696
Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter.
Zhang J, Yang Y, Dong Y, Liu C
International journal of molecular medicine 2022 Feb;49(2)
International journal of molecular medicine 2022 Feb;49(2)
Lycorine upregulates the expression of RMB10, promotes apoptosis and inhibits the proliferation and migration of cervical cancer cells.
Li Z, Zhou Q, Liu X, Li Y, Fan X, Liu G
International journal of molecular medicine 2022 Dec;50(6)
International journal of molecular medicine 2022 Dec;50(6)
LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer.
Piao HY, Guo S, Jin H, Wang Y, Zhang J
Journal of Cancer 2021;12(8):2336-2350
Journal of Cancer 2021;12(8):2336-2350
Isoflurane promotes epithelial-to-mesenchymal transition and metastasis of bladder cancer cells through HIF-1α-β-catenin/Notch1 pathways.
Lu N, Piao MH, Feng CS, Yuan Y
Life sciences 2020 Oct 1;258:118154
Life sciences 2020 Oct 1;258:118154
Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.
Shi R, Liu L, Wang F, He Y, Niu Y, Wang C, Zhang X, Zhang X, Zhang H, Chen M, Wang Y
Cellular signalling 2020 Dec;76:109810
Cellular signalling 2020 Dec;76:109810
Hsa_circ_001569 is an unfavorable prognostic factor and promotes cell proliferation and metastasis by modulating PI3K-AKT pathway in breast cancer.
Xu JH, Wang Y, Xu D
Cancer biomarkers : section A of Disease markers 2019;25(2):193-201
Cancer biomarkers : section A of Disease markers 2019;25(2):193-201
Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1.
Zhang J, Jin HY, Wu Y, Zheng ZC, Guo S, Wang Y, Yang D, Meng XY, Xu X, Zhao Y
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2019 Sep;21(9):1142-1151
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2019 Sep;21(9):1142-1151
Interleukin-27 Inhibits Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition in Preeclampsia.
Ge H, Yin N, Han TL, Huang D, Chen X, Xu P, He C, Tong C, Qi H
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jul;26(7):928-938
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jul;26(7):928-938
Immunomodulatory effect of human amniotic epithelial cells on restoration of ovarian function in mice with autoimmune ovarian disease.
Zhang Q, Huang Y, Sun J, Gu T, Shao X, Lai D
Acta biochimica et biophysica Sinica 2019 Aug 5;51(8):845-855
Acta biochimica et biophysica Sinica 2019 Aug 5;51(8):845-855
Up-regulated circular RNA hsa_circ_0067934 contributes to glioblastoma progression through activating PI3K-AKT pathway.
Xin J, Zhang XY, Sun DK, Tian LQ, Xu P
European review for medical and pharmacological sciences 2019 Apr;23(8):3447-3454
European review for medical and pharmacological sciences 2019 Apr;23(8):3447-3454
Hsa_circ_0005075 predicts a poor prognosis and acts as an oncogene in colorectal cancer via activating Wnt/β-catenin pathway.
Jin YD, Ren YR, Gao YX, Zhang L, Ding Z
European review for medical and pharmacological sciences 2019 Apr;23(8):3311-3319
European review for medical and pharmacological sciences 2019 Apr;23(8):3311-3319
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
Jia W, Jiang X, Liu W, Wang L, Zhu B, Zhu H, Liu X, Zhong M, Xie D, Huang W, Jia W, Li S, Liu X, Zuo X, Cheng D, Dai J, Ren C
International journal of oncology 2018 Jun;52(6):1787-1800
International journal of oncology 2018 Jun;52(6):1787-1800
Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Shen T, Cai LD, Liu YH, Li S, Gan WJ, Li XM, Wang JR, Guo PD, Zhou Q, Lu XX, Sun LN, Li JM
Journal of hematology & oncology 2018 Jul 17;11(1):95
Journal of hematology & oncology 2018 Jul 17;11(1):95
Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells.
Liang G, Fang X, Yang Y, Song Y
Acta histochemica 2018 Jan;120(1):56-63
Acta histochemica 2018 Jan;120(1):56-63
MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7.
Ping W, Gao Y, Fan X, Li W, Deng Y, Fu X
Biochemical and biophysical research communications 2018 Jan 22;495(4):2482-2489
Biochemical and biophysical research communications 2018 Jan 22;495(4):2482-2489
Anti-cancer effect of Aquaporin 5 silencing in colorectal cancer cells in association with inhibition of Wnt/β-catenin pathway.
Wang W, Li Q, Yang T, Li D, Ding F, Sun H, Bai G
Cytotechnology 2018 Apr;70(2):615-624
Cytotechnology 2018 Apr;70(2):615-624
Andrographolide Induces Autophagic Cell Death and Inhibits Invasion and Metastasis of Human Osteosarcoma Cells in An Autophagy-Dependent Manner.
Liu Y, Zhang Y, Zou J, Yan L, Yu X, Lu P, Wu X, Li Q, Gu R, Zhu D
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(4):1396-1410
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(4):1396-1410
E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin.
Huang L, Jiang J, Guo Q, Yang H
Molecular medicine reports 2017 Oct;16(4):5031-5035
Molecular medicine reports 2017 Oct;16(4):5031-5035
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma.
Zhang Y, Liu Y, Zou J, Yan L, Du W, Zhang Y, Sun H, Lu P, Geng S, Gu R, Zhang H, Bi Z
Oncotarget 2017 Oct 31;8(53):91134-91149
Oncotarget 2017 Oct 31;8(53):91134-91149
ANXA1 affects cell proliferation, invasion and epithelial-mesenchymal transition of oral squamous cell carcinoma.
Wan YM, Tian J, Qi L, Liu LM, Xu N
Experimental and therapeutic medicine 2017 Nov;14(5):5214-5218
Experimental and therapeutic medicine 2017 Nov;14(5):5214-5218
MgCl(2) and ZnCl(2) promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway.
Pan S, An L, Meng X, Li L, Ren F, Guan Y
Experimental and therapeutic medicine 2017 Nov;14(5):4663-4670
Experimental and therapeutic medicine 2017 Nov;14(5):4663-4670
Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage.
Zhang Q, Bu S, Sun J, Xu M, Yao X, He K, Lai D
Stem cell research & therapy 2017 Nov 28;8(1):270
Stem cell research & therapy 2017 Nov 28;8(1):270
Different miR-21-3p isoforms and their different features in colorectal cancer.
Jiao W, Leng X, Zhou Q, Wu Y, Sun L, Tan Y, Ni H, Dong X, Shen T, Liu Y, Li J
International journal of cancer 2017 Nov 15;141(10):2103-2111
International journal of cancer 2017 Nov 15;141(10):2103-2111
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition.
Lin L, Sun Y, Wang D, Zheng S, Zhang J, Zheng C
Frontiers in pharmacology 2015;6:320
Frontiers in pharmacology 2015;6:320
SOX9 Overexpression Promotes Glioma Metastasis via Wnt/β-Catenin Signaling.
Liu H, Liu Z, Jiang B, Peng R, Ma Z, Lu J
Cell biochemistry and biophysics 2015 Sep;73(1):205-12
Cell biochemistry and biophysics 2015 Sep;73(1):205-12
Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer.
Tong W, Sun D, Wang Q, Suo J
Cell biochemistry and biophysics 2015 Jun;72(2):453-9
Cell biochemistry and biophysics 2015 Jun;72(2):453-9
Expression of N-cadherin in myocardial tissues during the development of a rat heart.
Mu LM, Wang WF, Zheng H, Guo ZK, Zhang GM
Genetics and molecular research : GMR 2015 Aug 19;14(3):9882-9
Genetics and molecular research : GMR 2015 Aug 19;14(3):9882-9
Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/β-catenin pathway in laryngeal cancer Hep-2 cells.
Yang N, Hui L, Wang Y, Yang H, Jiang X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Aug;35(8):7965-73
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Aug;35(8):7965-73
Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin.
Wang SQ, Li XJ, Zhou S, Sun DX, Wang H, Cheng PF, Ma XR, Liu L, Liu JX, Wang FF, Liang YF, Wu JM
PloS one 2013;8(4):e61687
PloS one 2013;8(4):e61687
Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-beta1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 expressions.
Qiao B, Johnson NW, Gao J
International journal of oncology 2010 Sep;37(3):663-8
International journal of oncology 2010 Sep;37(3):663-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CDH2 using anti-CDH2 antibody (PA1328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: human SKOV3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # PA1328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140KD. The expected band size for CDH2 is at 100KD.
- Additional image