AF2957

antibody from Novus Biologicals

Targeting: S100P

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2957

Provider

Novus Biologicals

Product name

Goat Polyclonal S100P Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human S100P in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B and rhS100A10 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Morphological subclassification of intrahepatic cholangiocarcinoma: etiological, clinicopathological, and molecular features.

Liau JY, Tsai JH, Yuan RH, Chang CN, Lee HJ, Jeng YM
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2014 Aug;27(8):1163-73

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Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).

Shimamoto S, Kubota Y, Yamaguchi F, Tokumitsu H, Kobayashi R
The Journal of biological chemistry 2013 Mar 8;288(10):7158-68

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Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma.

Bartling B, Rehbein G, Simm A, Silber RE, Hofmann HS
International journal of oncology 2010 Apr;36(4):1015-21

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Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma.

Bartling B, Rehbein G, Simm A, Silber RE, Hofmann HS
International journal of oncology 2010 Apr;36(4):1015-21

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Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C.

Ludvigsen M, Jacobsen C, Maunsbach AB, Honoré B
Proteomics 2009 Dec;9(23):5267-87

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human S100P by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

S100P in Human Stomach. S100P in Human Stomach.