41-5100

antibody from Invitrogen Antibodies

Targeting: CLDN10 CPETRL3, OSP-L

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

41-5100

Provider

Invitrogen Antibodies

Product name

Anti-Claudin 10 Monoclonal Antibody (4B8A12)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

4B8A12

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

-20°C

References

Submitted references

Minimal effects of VEGF and anti-VEGF drugs on the permeability or selectivity of RPE tight junctions.

Peng S, Adelman RA, Rizzolo LJ
Investigative ophthalmology & visual science 2010 Jun;51(6):3216-25

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Molecular staging of epithelial maturation using secretory cell-specific genes as markers.

Zemke AC, Snyder JC, Brockway BL, Drake JA, Reynolds SD, Kaminski N, Stripp BR
American journal of respiratory cell and molecular biology 2009 Mar;40(3):340-8

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Notch signaling controls the balance of ciliated and secretory cell fates in developing airways.

Tsao PN, Vasconcelos M, Izvolsky KI, Qian J, Lu J, Cardoso WV
Development (Cambridge, England) 2009 Jul;136(13):2297-307

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence imaging analysis of mouse lung tissue cryosection using mouse anti-claudin-10 antibody (Catalog no. 41-5100) (green fluorescence) and rabbit anti-ZO-1 antibody (red fluorescence). Nuclei are stained with DAPI (blue fluorescence).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Claudin-10 showing staining in the membrane of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-10 monoclonal antibody (Product # 41-5100) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.