- MA5-17174 - Invitrogen Antibodies SKP1 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SKP1 using a SKP1 monoclonal antibody (Product # MA5-17174) against a human SKP1 (AA: 1-160) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SKP1 using SKP1 monoclonal antibody (Product # MA5-17174) in HeLa (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and COS-7 (7) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SKP1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SKP1 Monoclonal Antibody (1H9) (Product # MA5-17174) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytosolic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The knockdown of SKP1 was achieved by transfecting U-87 MG cells with SKP1 specific siRNA (Silencer® select Product # S12890, S12891). Immunofluorescence analysis was performed on untransfected U-87 MG cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e), and transfected with SKP1 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labeled with SKP1 Monoclonal Antibody (1H9) (Product # MA5-17174, 1:100 dilution) followed by Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. The reduction of the specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to SKP1 (Green). The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 monoclonal antibody (Product # MA5-17174) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 monoclonal antibody (Product # MA5-17174) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of HeLa cells using SKP1 monoclonal antibody (Product # MA5-17174) (green) and negative control (red).

MA5-17174