669801

antibody from BioLegend

Targeting: SKP1 EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

669801

Provider

BioLegend

Product name

Purified anti-Skp1

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W18108A

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Skp1 antibody (clone W18108A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.

Conjugate

Unconjugated