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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- 14-9699-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAR2 Monoclonal Antibody (SAM11), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody SAM11 recognizes human PAR2 (Protease activated receptor 2). PAR2 is a member of the PAR family which is a sub-family of the G protein coupled receptors (GPCR). Unlike most of the GPCR family members, PARs are not activated through ligand binding, but through proteolytic cleavage of the amino terminus. PAR2 activation is induced by trypsin, mast cell tryptase, neutrophil proteinase 3, tissue factor-factor VIIa-factor Xa, human kallikrein-related peptidases and membrane-tethered serine proteinase-1/matriptase-1. Unlike other PARs, PAR2 is not activated by thrombin. PAR2 is widely expressed in many tissues, including skin, liver, kidney, small intestine, and colon. PAR2 plays a key role in numerous inflammatory diseases and cancers. The SAM11 antibody has been reported to inhibit the ability of serine proteinases to activate PAR2.
- Antibody clone number
- SAM11
- Concentration
- 0.5 mg/mL
Submitted references Evaluation of antibodies directed against human protease-activated receptor-2.
PAR2 absence completely rescues inflammation and ichthyosis caused by altered CAP1/Prss8 expression in mouse skin.
Mucosal exposure to cockroach extract induces allergic sensitization and allergic airway inflammation.
Met receptor tyrosine kinase transactivation is involved in proteinase-activated receptor-2-mediated hepatocellular carcinoma cell invasion.
Role of matriptase and proteinase-activated receptor-2 in nonmelanoma skin cancer.
Agonists of proteinase-activated receptor-2 stimulate upregulation of intercellular cell adhesion molecule-1 in primary human keratinocytes via activation of NF-kappa B.
Adams MN, Pagel CN, Mackie EJ, Hooper JD
Naunyn-Schmiedeberg's archives of pharmacology 2012 Sep;385(9):861-73
Naunyn-Schmiedeberg's archives of pharmacology 2012 Sep;385(9):861-73
PAR2 absence completely rescues inflammation and ichthyosis caused by altered CAP1/Prss8 expression in mouse skin.
Frateschi S, Camerer E, Crisante G, Rieser S, Membrez M, Charles RP, Beermann F, Stehle JC, Breiden B, Sandhoff K, Rotman S, Haftek M, Wilson A, Ryser S, Steinhoff M, Coughlin SR, Hummler E
Nature communications 2011 Jan 18;2:161
Nature communications 2011 Jan 18;2:161
Mucosal exposure to cockroach extract induces allergic sensitization and allergic airway inflammation.
Arizmendi NG, Abel M, Puttagunta L, Asaduzzaman M, Davidson C, Karimi K, Forsythe P, Vliagoftis H
Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 2011 Dec 14;7:22
Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 2011 Dec 14;7:22
Met receptor tyrosine kinase transactivation is involved in proteinase-activated receptor-2-mediated hepatocellular carcinoma cell invasion.
Kaufmann R, Oettel C, Horn A, Halbhuber KJ, Eitner A, Krieg R, Katenkamp K, Henklein P, Westermann M, Böhmer FD, Ramachandran R, Saifeddine M, Hollenberg MD, Settmacher U
Carcinogenesis 2009 Sep;30(9):1487-96
Carcinogenesis 2009 Sep;30(9):1487-96
Role of matriptase and proteinase-activated receptor-2 in nonmelanoma skin cancer.
Bocheva G, Rattenholl A, Kempkes C, Goerge T, Lin CY, D'Andrea MR, Ständer S, Steinhoff M
The Journal of investigative dermatology 2009 Jul;129(7):1816-23
The Journal of investigative dermatology 2009 Jul;129(7):1816-23
Agonists of proteinase-activated receptor-2 stimulate upregulation of intercellular cell adhesion molecule-1 in primary human keratinocytes via activation of NF-kappa B.
Buddenkotte J, Stroh C, Engels IH, Moormann C, Shpacovitch VM, Seeliger S, Vergnolle N, Vestweber D, Luger TA, Schulze-Osthoff K, Steinhoff M
The Journal of investigative dermatology 2005 Jan;124(1):38-45
The Journal of investigative dermatology 2005 Jan;124(1):38-45
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PAR2 Monoclonal Antibody (Product # 14-9699-82) and 58, 55 kDa bands corresponding to PAR2 were observed across the cell lines and tissues tested along with an uncharacterized band at ~30 kDa. Membrane enriched extracts (40 µg lysate) of HCT 116 (Lane 1), PANC-1 (Lane 2), Hep G2 (Lane 3), A549 (Lane 4), OVCAR-3 (Lane 5), Daudi (Lan3 6), tissue extracts of Mouse Lung (Lane 7) and Mouse Skin (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:250 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized A549 cells using 10 µg/mL of Anti-Human PAR2 Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human skin tissue using 10 µg/mL of Anti-Human PAR2 Purified followed by Anti-Mouse IgG Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
