NBP1-28700

antibody from Novus Biologicals

Targeting: BTF3 BTF3a, BTF3b, NACB

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-28700

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-28700, RRID:AB_1913922

Product name

Rabbit Polyclonal BTF3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: BTF3 Antibody [NBP1-28700] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody NBP1-28700 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody NBP1-28716, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, NBP1-28716 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: BTF3 Antibody [NBP1-28700] - Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- BTF3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB