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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB8999 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR8 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9NR97- Isotype
- IgG
- Antibody clone number
- 935166
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references FcγRIIIa Signaling Modulates Endosomal TLR Responses in Human CD4+ T Cells.
Chauhan AK
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4596-4606
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4596-4606
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TLR8 and eGFP was stained with and either (A) Mouse Anti-Human TLR8 Monoclonal Antibody (Catalog # MAB8999) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
