Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB0089 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0089, RRID:AB_1017019
- Product name
- TLR8 monoclonal antibody, clone 44C143
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of TLR8.
- Isotype
- IgG
- Antibody clone number
- 44C143
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
CD molecules 2006--human cell differentiation molecules.
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
CD molecules 2006--human cell differentiation molecules.
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Lee J, Wu CC, Lee KJ, Chuang TH, Katakura K, Liu YT, Chan M, Tawatao R, Chung M, Shen C, Cottam HB, Lai MM, Raz E, Carson DA
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.
Levy O, Suter EE, Miller RL, Wessels MR
Blood 2006 Aug 15;108(4):1284-90
Blood 2006 Aug 15;108(4):1284-90
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production.
Hart OM, Athie-Morales V, O'Connor GM, Gardiner CM
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1636-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1636-42
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TLR8 in cell lysates from 293 transfected with human TLR8. Using TLR8 monoclonal antibody, clone 44C143 (Cat # MAB0089) at a concentration of 2 ug/mL .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR8 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) and TLR8 monoclonal antibody, clone 44C143 (Cat # MAB0089) (bottom) at 5 ug/mL .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR8 in 1x10^6 Ramos cells. Using 0.5 ug of TLR8 monoclonal antibody, clone 44C143 (Cat # MAB0089) histogram represents Ramos cells without antibody; Green represents isotype control (Sigma, Cat. No. M5284) ; red represents anti-TLR8 antibody.
- Validation comment
- Flow Cytometry
