ABIN1504659

antibody from antibodies-online

Targeting: SH3PXD2A FISH, KIAA0418, SH3MD1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1504659

Provider

antibodies-online

Product name

anti-SH3 and PX Domains 2A (SH3PXD2A) antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunocytochemistry Analysis (Representative lot data): Confocal fluorescent analysis of Mouse wild type and mutant 3T3-Src (Y527F) cells using a 1/1,000 dilution of TKS5 (SH3 #4) Antibody Cat.-No AP32620PU-N (Arrows indicate 3T3-Src (Y527F) cells expressing TKS5). Images were obtained with an Zeiss Axioplan 2 fluorescence microscope using a 40x magnification objective and processed with Axiovision 3.0 Software (Zeiss).

Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunoprecipitation: TKS5 was first immunoprecipitated from NIH/3T3 cell lysate using Anti-TKS5 (SH3 #4) Antibody Cat.-No AP32620PU-N, prior to western blotting analysis. Membranes were incubated with the indicated dilution of TKS5 Antibody Cat.-No AP32620PU-N for 1h at RT. Specific bands were detected by incubation with anti-rabbit-Alexa 680 (1/5,000) for 45' at RT. After washing in PBST, images were taken with an infrared imaging system.