12582-1-AP

antibody from Proteintech Group

Targeting: PTBP1 HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4

Provider product page for 12582-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 12582-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12582-1-AP, RRID:AB_2176402
Product name: PTBP1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PTBP1 antibody (Cat. #12582-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PTBP1 protein structure - 12582-1-AP shown in red.

Submitted references Prognostic Value and Biological Functions of RNA Binding Proteins in Stomach Adenocarcinoma.

Li J, Zhou W, Wei J, Xiao X, An T, Wu W, He Y
OncoTargets and therapy 2021;14:1689-1705

Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.

Chen J, Wu Y, Luo X, Jin D, Zhou W, Ju Z, Wang D, Meng Q, Wang H, Fu X, Xu J, Song Z
Theranostics 2021;11(15):7507-7526

Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.

Wu H, Cui M, Li C, Li H, Dai Y, Cui K, Li Z
Journal of agricultural and food chemistry 2021 Mar 17;69(10):3060-3068

Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy.

Liu J, He J, Ge L, Xiao H, Huang Y, Zeng L, Jiang Z, Lu M, Hu Z
Stem cell research & therapy 2021 Jul 22;12(1):413

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Ma Y, Liu S, Gao J, Chen C, Zhang X, Yuan H, Chen Z, Yin X, Sun C, Mao Y, Zhou F, Shao Y, Liu Q, Xu J, Cheng L, Yu D, Li P, Yi P, He J, Geng G, Guo Q, Si Y, Zhao H, Li H, Banes GL, Liu H, Nakamura Y, Kurita R, Huang Y, Wang X, Wang F, Fang G, Engel JD, Shi L, Zhang YE, Yu J
Developmental cell 2021 Feb 22;56(4):478-493.e11

LncRNA MIR155HG Promotes Temozolomide Resistance by Activating the Wnt/β-Catenin Pathway Via Binding to PTBP1 in Glioma.

He X, Sheng J, Yu W, Wang K, Zhu S, Liu Q
Cellular and molecular neurobiology 2021 Aug;41(6):1271-1284

Zika virus dysregulates the expression of astrocytic genes involved in neurodevelopment.

Shereen MA, Bashir N, Su R, Liu F, Wu K, Luo Z, Wu J
PLoS neglected tropical diseases 2021 Apr;15(4):e0009362

p53-targeted lncRNA ST7-AS1 acts as a tumour suppressor by interacting with PTBP1 to suppress the Wnt/β-catenin signalling pathway in glioma.

Sheng J, He X, Yu W, Chen Y, Long Y, Wang K, Zhu S, Liu Q
Cancer letters 2021 Apr 10;503:54-68

Abnormal PTBP1 Expression Sustains the Disease Progression of Multiple Myeloma.

Bai H, Chen B
Disease markers 2020;2020:4013658

hnRNP K Is a Novel Internal Ribosomal Entry Site-Transacting Factor That Negatively Regulates Foot-and-Mouth Disease Virus Translation and Replication and Is Antagonized by Viral 3C Protease.

Liu W, Yang D, Sun C, Wang H, Zhao B, Zhou G, Yu L
Journal of virology 2020 Aug 17;94(17)

circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.

Sun YM, Wang WT, Zeng ZC, Chen TQ, Han C, Pan Q, Huang W, Fang K, Sun LY, Zhou YF, Luo XQ, Luo C, Du X, Chen YQ
Blood 2019 Oct 31;134(18):1533-1546

Long noncoding RNA ANCR inhibits the differentiation of mesenchymal stem cells toward definitive endoderm by facilitating the association of PTBP1 with ID2.

Li J, Yang Y, Fan J, Xu H, Fan L, Li H, Zhao RC
Cell death & disease 2019 Jun 24;10(7):492

Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis.

Li H, Shen S, Ruan X, Liu X, Zheng J, Liu Y, Yang C, Wang D, Liu L, Ma J, Ma T, Wang P, Cai H, Li Z, Zhao L, Xue Y
Cell death & disease 2019 Dec 20;10(12):960

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Azam S, Hou S, Zhu B, Wang W, Hao T, Bu X, Khan M, Lei H
RNA biology 2019 Aug;16(8):1001-1009

SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein-RNA interactions with high accuracy.

Zhao Y, Zhang Y, Teng Y, Liu K, Liu Y, Li W, Wu L
Nucleic acids research 2019 Apr 8;47(6):e33

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Pospiech N, Cibis H, Dietrich L, Müller F, Bange T, Hennig S
Scientific reports 2018 Feb 12;8(1):2798

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Li Z, Zhou M, Cai Z, Liu H, Zhong W, Hao Q, Cheng D, Hu X, Hou J, Xu P, Xue Y, Zhou Y, Xu T
Nucleic acids research 2018 Dec 14;46(22):12052-12066

TRMP, a p53-inducible long noncoding RNA, regulates G1/S cell cycle progression by modulating IRES-dependent p27 translation.

Yang Y, Wang C, Zhao K, Zhang G, Wang D, Mei Y
Cell death & disease 2018 Aug 30;9(9):886

Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.

Liang X, Shi H, Yang L, Qiu C, Lin S, Qi Y, Li J, Zhao A, Liu J
British journal of cancer 2017 Mar 28;116(7):903-911

Activation of the intronic cryptic 5' splice site depends on its distance to the upstream cassette exon.

Liu W, Li X, Liao S, Dou K, Zhang Y
Gene 2017 Jul 1;619:30-36

LncRNA-MIF, a c-Myc-activated long non-coding RNA, suppresses glycolysis by promoting Fbxw7-mediated c-Myc degradation.

Zhang P, Cao L, Fan P, Mei Y, Wu M
EMBO reports 2016 Aug;17(8):1204-20

HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells.

Guo R, Li Y, Ning J, Sun D, Lin L, Liu X
PloS one 2013;8(4):e62729

Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.

Markus MA, Marques FZ, Morris BJ
PloS one 2011;6(12):e28926

Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes.

Gallagher IJ, Scheele C, Keller P, Nielsen AR, Remenyi J, Fischer CP, Roder K, Babraj J, Wahlestedt C, Hutvagner G, Pedersen BK, Timmons JA
Genome medicine 2010 Feb 1;2(2):9

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 12582-1-AP(PTBP1 antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of A2780 cells, using PTBP1 antibody 12582-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 12582-1-AP(PTBP1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue