630101

antibody from BioLegend

Targeting: PTBP1 HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

630101

Provider

BioLegend

Proper citation

BioLegend Cat#630101, RRID:AB_2171285

Product name

Purified anti-PTBP1

Antibody type

Monoclonal

Antigen

Recombinant human PTBP1 (full length)

Description

The 3H7 monoclonal antibody recognizes human PTBP1 also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57 kD RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6 kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5 kb. The 3H7 antibody has been shown to be useful for Western blotting.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Heat shock transcription factor Hsf1 is involved in tumor progression via regulation of hypoxia-inducible factor 1 and RNA-binding protein HuR.

Gabai VL, Meng L, Kim G, Mills TA, Benjamin IJ, Sherman MY
Molecular and cellular biology 2012 Mar;32(5):929-40

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Extracts from human PBMC were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against PTBP1 (clone 3H7). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Conjugate

Unconjugated