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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- AF6319 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal TEX19 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TEX19 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human TEX11 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis.
Planells-Palop V, Hazazi A, Feichtinger J, Jezkova J, Thallinger G, Alsiwiehri NO, Almutairi M, Parry L, Wakeman JA, McFarlane RJ
Molecular cancer 2017 Apr 26;16(1):84
Molecular cancer 2017 Apr 26;16(1):84
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human TEX19 by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEX19 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
