PAB27996

antibody from Abnova Corporation

Targeting: C3orf33 AC3-33, FLJ31139

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27996

Provider

Abnova Corporation

Product name

C3orf33 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C3orf33

Antigen sequence

RLTSKFTSSSDIPVEFIRRNVKLRGRLRRITENGL
EIEHIPITLPIIASLRKEPRGALLVKLAGVELAET
GKAWLQKELKPSQLLWFQL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4 Lane 2: U-251 MG Lane 3: Human Plasma Lane 4: Liver Lane 5: Tonsil with C3orf33 polyclonal antibody ( Cat # PAB27996 ) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with C3orf33 polyclonal antibody ( Cat # PAB27996 ) at 1-4 ug/ml dilution shows positivity in nucleus but not nucleoli & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human vulva/anal skin with C3orf33 polyclonal antibody ( Cat # PAB27996 ) shows strong nuclear positivity in squamous epithelial cells at 1:20 - 1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)