HPA036305

antibody from Atlas Antibodies

Targeting: IGSF3 EWI-3, MGC117164, V8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA036305

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA036305, RRID:AB_2675050

Product name

Anti-IGSF3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VGTVEFHDLVTFTRDGGVQWGDRSSSFRTRTAIEK
AESSNNVRLSISRASDTEAGKYQCVAELWRKNYNN
TWTRLAERTSNLLEIRVLQPVTKLQVSKSKRTLTL
VENKPIQLNCSVKSQTSQNSHFAVLWYVHKPSDA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines MCF-7 and HEK293 using Anti-IGSF3 antibody. Corresponding IGSF3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong membranous positivity in syncytiotrophoblastic cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong positivity in plasma membrane in trophoblastic cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Sample type

HUMAN