MA5-24216

antibody from Invitrogen Antibodies

Targeting: IGSF3 EWI-3, MGC117164, V8

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24216

Provider

Invitrogen Antibodies

Product name

IGSF3 Monoclonal Antibody (503621)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant human (rh) IGSF4, 4B, 4C, 4D or 8 is observed.

Antibody clone number

503621

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of mouse Anti-human IGSF3 Monoclonal Antibody (Product # MA5-24216) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IGSF3 in human lung tissue. Samples were incubated in IGSF3 monoclonal antibody (Product # MA5-24216) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of A549 human lung carcinoma cell line was stained with mouse Anti-human IGSF3 Monoclonal Antibody (Product # MA5-24216, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of IGSF3 in A549 human lung carcinoma cell line. Samples were incubated in IGSF3 monoclonal antibody (Product # MA5-24216) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody.