AF1379

antibody from R&D Systems

Targeting: REG4 GISP, REG-IV, RELP

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1379

Provider

R&D Systems

Product name

Human Reg4 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Reg4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) Reg4, rmReg3A, and recombinant rat (rr) Reg3A is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9BYZ8


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

ARTS mediates apoptosis and regeneration of the intestinal stem cell niche.

Koren E, Yosefzon Y, Ankawa R, Soteriou D, Jacob A, Nevelsky A, Ben-Yosef R, Bar-Sela G, Fuchs Y
Nature communications 2018 Nov 2;9(1):4582

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IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth.

Ayars M, O'Sullivan E, Macgregor-Das A, Shindo K, Kim H, Borges M, Yu J, Hruban RH, Goggins M
Oncotarget 2017 Oct 13;8(48):83370-83383

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Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon.

Sasaki N, Sachs N, Wiebrands K, Ellenbroek SI, Fumagalli A, Lyubimova A, Begthel H, van den Born M, van Es JH, Karthaus WR, Li VS, López-Iglesias C, Peters PJ, van Rheenen J, van Oudenaarden A, Clevers H
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 13;113(37):E5399-407

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RNA sequencing of sessile serrated colon polyps identifies differentially expressed genes and immunohistochemical markers.

Delker DA, McGettigan BM, Kanth P, Pop S, Neklason DW, Bronner MP, Burt RW, Hagedorn CH
PloS one 2014;9(2):e88367

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REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells.

Rafa L, Dessein AF, Devisme L, Buob D, Truant S, Porchet N, Huet G, Buisine MP, Lesuffleur T
International journal of oncology 2010 Mar;36(3):689-98

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REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells.

Rafa L, Dessein AF, Devisme L, Buob D, Truant S, Porchet N, Huet G, Buisine MP, Lesuffleur T
International journal of oncology 2010 Mar;36(3):689-98

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Reg IV is a serum biomarker for gastric cancer patients and predicts response to 5-fluorouracil-based chemotherapy.

Mitani Y, Oue N, Matsumura S, Yoshida K, Noguchi T, Ito M, Tanaka S, Kuniyasu H, Kamata N, Yasui W
Oncogene 2007 Jun 28;26(30):4383-93

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Reg4 in Human Colon Cancer Tissue. Reg4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Reg4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1379) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.