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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10201 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10201, RRID:AB_1671710
- Product name
- DIAPH2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DIAPH2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and characterization of human FMNL1, FMNL2 and FMNL3 genes in silico.
A human homologue of the Drosophila melanogaster diaphanous gene is disrupted in a patient with premature ovarian failure: evidence for conserved function in oogenesis and implications for human sterility.
Nonsyndromic deafness DFNA1 associated with mutation of a human homolog of the Drosophila gene diaphanous.
Katoh M, Katoh M
International journal of oncology 2003 May;22(5):1161-8
International journal of oncology 2003 May;22(5):1161-8
A human homologue of the Drosophila melanogaster diaphanous gene is disrupted in a patient with premature ovarian failure: evidence for conserved function in oogenesis and implications for human sterility.
Bione S, Sala C, Manzini C, Arrigo G, Zuffardi O, Banfi S, Borsani G, Jonveaux P, Philippe C, Zuccotti M, Ballabio A, Toniolo D
American journal of human genetics 1998 Mar;62(3):533-41
American journal of human genetics 1998 Mar;62(3):533-41
Nonsyndromic deafness DFNA1 associated with mutation of a human homolog of the Drosophila gene diaphanous.
Lynch ED, Lee MK, Morrow JE, Welcsh PL, León PE, King MC
Science (New York, N.Y.) 1997 Nov 14;278(5341):1315-8
Science (New York, N.Y.) 1997 Nov 14;278(5341):1315-8
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using DIAPH2 polyclonal antibody (Cat # PAB10201) shows detection of a 132 KDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells.Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody.Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.Achemiluminescence system was used forsignal detection using a 1 min exposure time.
