Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- 66269-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- L2HGDH Monoclonal Antibody (3D11C2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MVPALRYLV GACGRARGRF AGGSPGACGF ASGRPRPLCG GSRSASTSSF DIVIVGGGIV GLASARALIL RHPSLSIGVL EKEKDLAVHQ TGHNSGVIHS GIYYKPESLK AKLCVQGAAL LYEYCQQKGI SYKQCGKLIV AVEQEEIPRL QALYEKGLQN GVPGLRLIQQ EDIKKKEPYC RGLMAIDCPH TGIVDYRQVA LSFAQDFQEA GGSVLTNFEV KGIEMAKESP SRSIDGMQYP IVIKNTKGEE IRCQYVVTCA GLYSDRISEL SGCTPDPRIV PFRGDYLLLK PEKCYLVKGN IYPVPDSRFP FLGVHFTPRM DGSIWLGPNA VLAFKREGYR PFDFSATDVM DI (1-351 aa encoded by BC006117)
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D11C2
- Vial size
- 150 µL
- Concentration
- 1.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig brain tissue were subjected to SDS PAGE followed by western blot with 66269-1-IG ( L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66269-1-IG ( L2HGDH Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66269-1-IG ( L2HGDH Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (Product # 66269-1-IG, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).