11332-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF2B4 DKFZP586J0119, EIF-2B, EIF2B, EIF2Bdelta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11332-1-AP

Provider

Invitrogen Antibodies

Product name

EIF2B4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAVAVAVR EDSGSGMKAE LPPGPGAVGR EMTKEEKLQL RKEKKQQKKK RKEEKGAEPE TGSAVSAAQC QVGPTRELPE SGIQLGTPRE KVPAGRSKAE LRAERRAKQE AERALKQARK GEQGGPPPKA SPSTAGETPS GVKRLPEYPQ VDDLLLRRLV KKPERQQVPT RKDYGSKVSL FSHLPQYSRQ NSLTQFMSIP SSVIHPAMVR LGLQYSQGLV SGSNARCIAL LRALQQVIQD YTTPPNEELS RDLVNKLKPY MSFLTQCRPL SASMHNAIKF LNKEITSVGS SKREEEAKSE LRAAIDRYVQ EKIVLAAQAI SRFAYQKISN GDVILVYGCS SL (1-341 aa encoded by BC001870)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 11332-1-AP (EIF2B4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 11332-1-AP (EIF2B4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11332-1-AP (EIF2B4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis slide using 11332-1-AP (EIF2B4 Antibody) at dilution of 1:50.