Explore
Validate
LearnA302-982A
antibody from Invitrogen Antibodies
Targeting: EIF2B4
DKFZP586J0119, EIF-2B, EIF2B, EIF2Bdelta
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A302-982A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF2B delta/EIF2B4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human eIF2B delta/EIF2B4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2B delta/EIF2B4 antibody A302-982A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. eIF2B delta/EIF2B4 was also immunoprecipitated by rabbit anti-eIF2B delta/EIF2B4 antibody A302-983A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human eIF2B delta/EIF2B4 by immunohistochemistry. Samples: FFPE sections of human colon carcinoma and breast carcinoma. Antibody: Affinity purified rabbit anti- eIF2B delta/EIF2B4 (Cat. No. A302-982A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
