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LearnH00001756-M07
antibody from Abnova Corporation
Targeting: DMD
BMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00001756-M07 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001756-M07, RRID:AB_1136923
- Product name
- DMD monoclonal antibody (M07), clone 3A11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant DMD.
- Antigen sequence
MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRF
SAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNL
KQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPL
CVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCK
AHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQ
IPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEA
ALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKC
NICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAK
GHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTK
RYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPV
DSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGS
YLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQP
RSPAQILISLESEERGELERILADLEEENRNLQAE
YDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAEL
IAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRL
RQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPML
LRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVMEQL
NNSFPSSRGHNVGSLFHMADDLGRAMESLVSVMTD
EEGAE- Isotype
- IgG
- Antibody clone number
- 3A11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DMD expression in transfected 293T cell line by DMD monoclonal antibody (M07), clone 3A11.Lane 1: DMD transfected lysate(72.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DMD is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of DMD transfected lysate using anti-DMD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DMD MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
