PA5-57995
antibody from Invitrogen Antibodies
Targeting: SPAG16
DKFZp666P1710, FLJ22724, PF20, WDR29
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-57995 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPAG16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NLKKDLKHYK QAADKAREDL LKIQKERDFH RMHHKRIVQE KNKLINDLKG LKLHYASYEP TIRVLHEKHH TLLKEKMLTS LERDKVVG
- Concentration
- 0.4 mg/mL
Submitted references Deleterious variants in X-linked CFAP47 induce asthenoteratozoospermia and primary male infertility.
Liu C, Tu C, Wang L, Wu H, Houston BJ, Mastrorosa FK, Zhang W, Shen Y, Wang J, Tian S, Meng L, Cong J, Yang S, Jiang Y, Tang S, Zeng Y, Lv M, Lin G, Li J, Saiyin H, He X, Jin L, Touré A, Ray PF, Veltman JA, Shi Q, O'Bryan MK, Cao Y, Tan YQ, Zhang F
American journal of human genetics 2021 Feb 4;108(2):309-323
American journal of human genetics 2021 Feb 4;108(2):309-323
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG16 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a SPAG16 Polyclonal Antibody (Product # PA5-57995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG16 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a SPAG16 Polyclonal Antibody (Product # PA5-57995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG16 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a SPAG16 Polyclonal Antibody (Product # PA5-57995).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SPAG16 in human cell line A-431 shows positivity in cytoplasm. Samples were probed using a SPAG16 Polyclonal Antibody (Product # PA5-57995).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SPAG16 in human cell line A-431 using a SPAG16 Polyclonal Antibody (Product # PA5-57995) shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPAG16 in human breast tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a SPAG16 Polyclonal Antibody (Product # PA5-57995).