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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- AF829 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NAIP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human NAIP expressed in Sf 21 insect cells.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NP_004527- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils.
Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
European journal of immunology 2006 Jul;36(7):1975-84
European journal of immunology 2006 Jul;36(7):1975-84
Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils.
Altznauer F, Martinelli S, Yousefi S, Thürig C, Schmid I, Conway EM, Schöni MH, Vogt P, Mueller C, Fey MF, Zangemeister-Wittke U, Simon HU
The Journal of experimental medicine 2004 May 17;199(10):1343-54
The Journal of experimental medicine 2004 May 17;199(10):1343-54
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human NAIP by Western Blot. Western blot shows lysates of Sf 21 insect cells before (lane 1) and 48 hours post infection with human NAIP (lane 2). PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human NAIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF829) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for truncated NAIP was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
