PAB29444

antibody from Abnova Corporation

Targeting: PARP2 ADPRTL2, ARTD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29444

Provider

Abnova Corporation

Product name

PARP2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human PARP2.

Antigen sequence

ARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESK
KMPVAGGKANKDRTEDKQDESVKALLLKGKAPVDP
ECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKY
YLIQLLEDDAQRNFSVWM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of U-2 OS with PARP2 polyclonal antibody (Cat # PAB29444) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line MCF7 with PARP2 polyclonal antibody (Cat # PAB29444) at 1-4 ug/mL concentration shows positivity in nucleus.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with PARP2 polyclonal antibody (Cat # PAB29444) shows moderate nuclear positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)