39743

antibody from Invitrogen Antibodies

Targeting: PARP2 ADPRTL2, ARTD2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

39743

Provider

Invitrogen Antibodies

Product name

PARP-2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PARP-2 antibody tested by Western blot. Lane 1: U2OS nuclear extract (20 µg). Lane 2: A549 whole-cell extract(20 µg). Lane 3: Hep G2 whole-cell extract (20 µg). Lane 4: Jurkat cell nuclear extract (20 µg). These were probed with PARP-2 antibody at a dilution of 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PARP-2 antibody used for immunoprecipitation. Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lysate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.