24034-1-AP

antibody from Invitrogen Antibodies

Targeting: ANKRD27 DKFZp434L0718, FLJ00040, VARP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24034-1-AP

Provider

Invitrogen Antibodies

Product name

ANKRD27 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VTSQDGSSP LHVAALHGRA DLIPLLLKHG ANAGARNADQ AVPLHLACQQ GHFQVVKCLL DSNAKPNKKD LSGNTPLIYA CSGGHHELVA LLLQHGASIN ASNNKGNTAL HEAVIEKHVF VVELLLLHGA SVQVLNKRQR TAVDCAEQNS KIMELLQVVP SCVASLDDVA ETDRKEYVTV KIRKKWNSKL YDLPDEPFTR QFYFVHSAGQ FKGKTSREIM ARDRSVPNLT EGSLHEPGRQ SVTLRQNNLP AQSGSHAAEK GNSDWPERPG LTQTGPGHRR MLRRHTVEDA VVSQGPEAAG PLSTPQEVSA SRS (739-1050 aa encoded by B C050529)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 24034-1-AP ( ANKRD27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 24034-1-AP (ANKRD27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 24034-1-AP (ANKRD27 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 24034-1-AP ( ANKRD27 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ANKRD27 (IP:24034-1-AP, 4ug; Detection:24034-1-AP 1:1000) with A431 cells lysate 2000ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ANKRD27 (IP:24034-1-AP, 4 ug; Detection:24034-1-AP 1:500) with MCF-7 cells lysate 3200 ug.