Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP53324PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53324PU-N, RRID:AB_11148261
- Product name
- anti PLA2G2D (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 112-138 amino acids from the C-terminal region of human PLA2G2D
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PLA2G2D Antibody (C-term) Cat.-No AP53324PU-NÂ in HL-60 cell line lysates (35ug/lane). This demonstrates the PLA2G2D antibody detected the PLA2G2D protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancrease tissue reacted with PLA2G2D Antibody (C-term) Cat.-No AP53324PU-N, which peroxidase conjugated to the secondary antibody and was followed by DAB staining. This data demonstrates the use of PLA2G2D Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HL-60 cells using PLA2G2D Antibody (C-term) Cat.-No AP53324PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.