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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 25598-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFU1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAATARRGW GAAAVAAGLR RRFCHMLKNP YTIKKQPLHQ FVQRPLFPLP AAFYHPVRYM FIQTQDTPNP NSLKFIPGKP VLETRTMDFP TPAAAFRSPL ARQLFRIEGV KSVFFGPDFI TVTKENEELD WNLLKPDIYA TIMDFFASGL PLVTEETPSG EAGSEEDDEV VAMIKELLDT RIRPTVQEDG GDVIYKGFED GIVQLKLQGS CTSCPSSIIT LKNGIQNMLQ FYIPEVEGVE QVMDDESDEK EANSP (1-254 aa encoded by BC113692)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP ( NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
