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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-25628 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Vitamin B3, nicotinamide, enhances mitochondrial metabolism to promote differentiation of the retinal pigment epithelium.
Hazim RA, Paniagua AE, Tang L, Yang K, Kim KKO, Stiles L, Divakaruni AS, Williams DS
The Journal of biological chemistry 2022 Sep;298(9):102286
The Journal of biological chemistry 2022 Sep;298(9):102286
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis in mouse heart tissue lysates (15 µg per lane) using a CRUM2 polyclonal antibody (Product # PA5-25628).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded mouse kidney tissue using a CRUM2 polyclonal antibody (Product # PA5-25628), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cellular morphology of ARPE-19 cells cultured in the presence or the absence of nicotinamide. A , brightfield micrographs of ARPE-19 cells cultured for 2 weeks in MEM or MEM-Nic. B , immunofluorescence micrographs of ZO-1 labeling in ARPE-19 cells cultured on laminin-coated Transwell inserts for 3 weeks in the same media as for A . Cells cultured in MEM-Nic exhibited compact and cobblestone morphology. C , phalloidin labeling showed cortical arrangement of actin filaments in cells cultured in MEM-Nic. Cells cultured in the absence of nicotinamide (MEM) contained actin filaments arranged in stress fibers. D , immunofluorescence micrographs of CRB2 labeling in ARPE-19 cells cultured on laminin-coated Transwell inserts for 3 weeks in the same media as for A . Cells differentiated in MEM-Nic showed higher expression of CRB2 as well as junctional localization at the apical plasma membrane. The scale bars represent 50 mum ( A ) and 20 mum ( B - D ). All images are representative from at least three independent experiments. CRB2, crumbs cell polarity complex component 2; MEM-Nic, nicotinamide-containing minimum essential medium; ZO-1, zonula occludens-1.
