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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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- Product number
- NB600-936 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-936, RRID:AB_10000656
- Product name
- Rabbit Polyclonal beta Tubulin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Simian, Xenopus, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TACAN Is an Ion Channel Involved in Sensing Mechanical Pain.
The expression and activity of thymidine kinase 1 and deoxycytidine kinase are modulated by hydrogen peroxide and nucleoside analogs.
Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.
Degradome of soluble ADAM10 and ADAM17 metalloproteases.
Nociceptin attenuates the escalation of oxycodone self-administration by normalizing CeA-GABA transmission in highly addicted rats.
Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance.
AAV Mediated Delivery of Myxoma Virus M013 Gene Protects the Retina against Autoimmune Uveitis.
Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina.
Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study.
Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor.
Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload.
Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein.
How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions.
Environmental Enrichment Reverses Tyrosine Kinase Inhibitor-Mediated Impairment Through BDNF-TrkB Pathway.
Nanodelivery of Cerebrolysin and Rearing in Enriched Environment Induce Neuroprotective Effects in a Preclinical Rat Model of Parkinson's Disease.
Deleterious Effects of VEGFR2 and RET Inhibition in a Preclinical Model of Parkinson's Disease.
Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models.
Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice.
C1QTNF3 in the murine ovary and its function in folliculogenesis.
Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival.
An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males.
Novel Anthra[1,2-c][1,2,5]Thiadiazole-6,11-Diones as Promising Anticancer Lead Compounds: Biological Evaluation, Characterization & Molecular Targets Determination.
Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression.
Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions.
3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer.
Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion.
Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers.
Effects of inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate induced insulin resistance in L6 myotubes.
Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
Probing relevant molecules in modulating the neurite outgrowth of hippocampal neurons on substrates of different stiffness.
3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer.
Increased IFNα activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits.
Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection.
NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia.
Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection.
THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis.
The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death.
Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability.
The genomic response of the retinal pigment epithelium to light damage and retinal detachment.
Beaulieu-Laroche L, Christin M, Donoghue A, Agosti F, Yousefpour N, Petitjean H, Davidova A, Stanton C, Khan U, Dietz C, Faure E, Fatima T, MacPherson A, Mouchbahani-Constance S, Bisson DG, Haglund L, Ouellet JA, Stone LS, Samson J, Smith MJ, Ask K, Ribeiro-da-Silva A, Blunck R, Poole K, Bourinet E, Sharif-Naeini R
Cell 2020 Mar 5;180(5):956-967.e17
Cell 2020 Mar 5;180(5):956-967.e17
The expression and activity of thymidine kinase 1 and deoxycytidine kinase are modulated by hydrogen peroxide and nucleoside analogs.
Sun R, Eriksson S, Wang L
Nucleosides, nucleotides & nucleic acids 2020 Mar 19;:1-12
Nucleosides, nucleotides & nucleic acids 2020 Mar 19;:1-12
Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.
Weinstock NI, Shin D, Dhimal N, Hong X, Irons EE, Silvestri NJ, Reed CB, Nguyen D, Sampson O, Cheng YC, Lau JTY, Bongarzone ER, Kofler J, Escolar ML, Gelb MH, Wrabetz L, Feltri ML
Neuron 2020 Jul 8;107(1):65-81.e9
Neuron 2020 Jul 8;107(1):65-81.e9
Degradome of soluble ADAM10 and ADAM17 metalloproteases.
Scharfenberg F, Helbig A, Sammel M, Benzel J, Schlomann U, Peters F, Wichert R, Bettendorff M, Schmidt-Arras D, Rose-John S, Moali C, Lichtenthaler SF, Pietrzik CU, Bartsch JW, Tholey A, Becker-Pauly C
Cellular and molecular life sciences : CMLS 2020 Jan;77(2):331-350
Cellular and molecular life sciences : CMLS 2020 Jan;77(2):331-350
Nociceptin attenuates the escalation of oxycodone self-administration by normalizing CeA-GABA transmission in highly addicted rats.
Kallupi M, Carrette LLG, Kononoff J, Solberg Woods LC, Palmer AA, Schweitzer P, George O, de Guglielmo G
Proceedings of the National Academy of Sciences of the United States of America 2020 Jan 28;117(4):2140-2148
Proceedings of the National Academy of Sciences of the United States of America 2020 Jan 28;117(4):2140-2148
Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance.
Gyöngyösi M, Lukovic D, Zlabinger K, Spannbauer A, Gugerell A, Pavo N, Traxler D, Pils D, Maurer G, Jakab A, Riesenhuber M, Pircher A, Winkler J, Bergler-Klein J
Cardiovascular research 2020 Apr 1;116(5):970-982
Cardiovascular research 2020 Apr 1;116(5):970-982
AAV Mediated Delivery of Myxoma Virus M013 Gene Protects the Retina against Autoimmune Uveitis.
Ridley RB, Young BM, Lee J, Walsh E, Ahmed CM, Lewin AS, Ildefonso CJ
Journal of clinical medicine 2019 Nov 29;8(12)
Journal of clinical medicine 2019 Nov 29;8(12)
Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina.
Lee BJ, Jun HO, Kim JH, Kim JH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6045-6054
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6045-6054
Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study.
Hannen R, Steffani M, Voellger B, Carl B, Wang J, Bartsch JW, Nimsky C
The Journal of steroid biochemistry and molecular biology 2019 Mar;187:88-96
The Journal of steroid biochemistry and molecular biology 2019 Mar;187:88-96
Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor.
Hannen R, Selmansberger M, Hauswald M, Pagenstecher A, Nist A, Stiewe T, Acker T, Carl B, Nimsky C, Bartsch JW
Cancers 2019 Jun 30;11(7)
Cancers 2019 Jun 30;11(7)
Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload.
Zhang T, Wu C, Yang X, Liu Y, Yang H, Yuan L, Liu Y, Sun S, Yang J
Neuroscience 2019 Jul 15;411:86-104
Neuroscience 2019 Jul 15;411:86-104
Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein.
Castle AR, Daude N, Gilch S, Westaway D
The Journal of biological chemistry 2019 Feb 22;294(8):2642-2650
The Journal of biological chemistry 2019 Feb 22;294(8):2642-2650
How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions.
Stuart JA, Fonseca J, Moradi F, Cunningham C, Seliman B, Worsfold CR, Dolan S, Abando J, Maddalena LA
Oxidative medicine and cellular longevity 2018;2018:8238459
Oxidative medicine and cellular longevity 2018;2018:8238459
Environmental Enrichment Reverses Tyrosine Kinase Inhibitor-Mediated Impairment Through BDNF-TrkB Pathway.
Bengoetxea H, Rico-Barrio I, Ortuzar N, Murueta-Goyena A, Lafuente JV
Molecular neurobiology 2018 Jan;55(1):43-59
Molecular neurobiology 2018 Jan;55(1):43-59
Nanodelivery of Cerebrolysin and Rearing in Enriched Environment Induce Neuroprotective Effects in a Preclinical Rat Model of Parkinson's Disease.
Requejo C, Ruiz-Ortega JA, Cepeda H, Sharma A, Sharma HS, Ozkizilcik A, Tian R, Moessler H, Ugedo L, Lafuente JV
Molecular neurobiology 2018 Jan;55(1):286-299
Molecular neurobiology 2018 Jan;55(1):286-299
Deleterious Effects of VEGFR2 and RET Inhibition in a Preclinical Model of Parkinson's Disease.
Requejo C, Ruiz-Ortega JA, Bengoetxea H, Bulnes S, Ugedo L, Lafuente JV
Molecular neurobiology 2018 Jan;55(1):201-212
Molecular neurobiology 2018 Jan;55(1):201-212
Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models.
Schulte ML, Fu A, Zhao P, Li J, Geng L, Smith ST, Kondo J, Coffey RJ, Johnson MO, Rathmell JC, Sharick JT, Skala MC, Smith JA, Berlin J, Washington MK, Nickels ML, Manning HC
Nature medicine 2018 Feb;24(2):194-202
Nature medicine 2018 Feb;24(2):194-202
Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice.
Yang L, Chen L, Lu X, Tan A, Chen Y, Li Y, Peng X, Yuan S, Cai D, Yu Y
Reproduction (Cambridge, England) 2018 Aug;156(2):133-144
Reproduction (Cambridge, England) 2018 Aug;156(2):133-144
C1QTNF3 in the murine ovary and its function in folliculogenesis.
Mao Z, Yang L, Lu X, Tan A, Wang Y, Ding F, Xiao L, Qi X, Yu Y
Reproduction (Cambridge, England) 2018 Apr;155(4):333-346
Reproduction (Cambridge, England) 2018 Apr;155(4):333-346
Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival.
Gelmedin V, Morel M, Hahnel S, Cailliau K, Dissous C, Grevelding CG
PLoS pathogens 2017 Jan;13(1):e1006147
PLoS pathogens 2017 Jan;13(1):e1006147
An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males.
Lau AC, Zhu KP, Brouhard EA, Davis MB, Csankovszki G
Epigenetics & chromatin 2016;9:44
Epigenetics & chromatin 2016;9:44
Novel Anthra[1,2-c][1,2,5]Thiadiazole-6,11-Diones as Promising Anticancer Lead Compounds: Biological Evaluation, Characterization & Molecular Targets Determination.
Ali AA, Lee YR, Chen TC, Chen CL, Lee CC, Shiau CY, Chiang CH, Huang HS
PloS one 2016;11(4):e0154278
PloS one 2016;11(4):e0154278
Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression.
Snyder MJ, Lau AC, Brouhard EA, Davis MB, Jiang J, Sifuentes MH, Csankovszki G
PLoS genetics 2016 Sep;12(9):e1006341
PLoS genetics 2016 Sep;12(9):e1006341
Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions.
Lee MC, Huang HJ, Chang TH, Huang HC, Hsieh SY, Chen YS, Chou WY, Chiang CH, Lai CH, Shiau CY
Scientific reports 2016 Jul 4;6:29311
Scientific reports 2016 Jul 4;6:29311
3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer.
McKinley ET, Zhao P, Coffey RJ, Washington MK, Manning HC
PloS one 2014;9(9):e108193
PloS one 2014;9(9):e108193
Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion.
Orlicky DJ, Monks J, Stefanski AL, McManaman JL
PloS one 2013;8(6):e66837
PloS one 2013;8(6):e66837
Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers.
Riva M, He Z, Källberg E, Ivars F, Leanderson T
PloS one 2013;8(4):e61832
PloS one 2013;8(4):e61832
Effects of inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate induced insulin resistance in L6 myotubes.
Mikłosz A, Łukaszuk B, Baranowski M, Górski J, Chabowski A
PloS one 2013;8(12):e85547
PloS one 2013;8(12):e85547
Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
Lu ZH, Kaliberov S, Sohn RE, Kaliberova L, Curiel DT, Arbeit JM
PloS one 2013;8(12):e83933
PloS one 2013;8(12):e83933
Probing relevant molecules in modulating the neurite outgrowth of hippocampal neurons on substrates of different stiffness.
Chen WH, Cheng SJ, Tzen JT, Cheng CM, Lin YW
PloS one 2013;8(12):e83394
PloS one 2013;8(12):e83394
3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer.
McKinley ET, Smith RA, Zhao P, Fu A, Saleh SA, Uddin MI, Washington MK, Coffey RJ, Manning HC
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2013 Mar;54(3):424-30
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2013 Mar;54(3):424-30
Increased IFNα activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits.
Jacek E, Fallon BA, Chandra A, Crow MK, Wormser GP, Alaedini A
Journal of neuroimmunology 2013 Feb 15;255(1-2):85-91
Journal of neuroimmunology 2013 Feb 15;255(1-2):85-91
Differences in iNOS and arginase expression and activity in the macrophages of rats are responsible for the resistance against T. gondii infection.
Li Z, Zhao ZJ, Zhu XQ, Ren QS, Nie FF, Gao JM, Gao XJ, Yang TB, Zhou WL, Shen JL, Wang Y, Lu FL, Chen XG, Hide G, Ayala FJ, Lun ZR
PloS one 2012;7(4):e35834
PloS one 2012;7(4):e35834
NEUROG3 is a critical downstream effector for STAT3-regulated differentiation of mammalian stem and progenitor spermatogonia.
Kaucher AV, Oatley MJ, Oatley JM
Biology of reproduction 2012 May;86(5):164, 1-11
Biology of reproduction 2012 May;86(5):164, 1-11
Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection.
Kadiu I, Gendelman HE
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2011 Dec;6(4):658-75
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2011 Dec;6(4):658-75
THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis.
Reding SC, Stepnoski AL, Cloninger EW, Oatley JM
Reproduction (Cambridge, England) 2010 May;139(5):893-903
Reproduction (Cambridge, England) 2010 May;139(5):893-903
The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death.
Lin SY, Chen NT, Sun SP, Chang JC, Wang YC, Yang CS, Lo LW
Journal of the American Chemical Society 2010 Jun 23;132(24):8309-15
Journal of the American Chemical Society 2010 Jun 23;132(24):8309-15
Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability.
Catalucci D, Zhang DH, DeSantiago J, Aimond F, Barbara G, Chemin J, Bonci D, Picht E, Rusconi F, Dalton ND, Peterson KL, Richard S, Bers DM, Brown JH, Condorelli G
The Journal of cell biology 2009 Mar 23;184(6):923-33
The Journal of cell biology 2009 Mar 23;184(6):923-33
The genomic response of the retinal pigment epithelium to light damage and retinal detachment.
Rattner A, Toulabi L, Williams J, Yu H, Nathans J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Sep 24;28(39):9880-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Sep 24;28(39):9880-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification].
