25671-1-AP

antibody from Proteintech Group

Targeting: CHCHD10 C22orf16, MIX17A, N27C7-4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25671-1-AP

Provider

Proteintech Group

Product name

CHCHD10 antibody

Antibody type

Polyclonal

Description

KD/KO validated CHCHD10 antibody (Cat. #25671-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Mouse midbrain dopaminergic neurons survive loss of the PD-associated mitochondrial protein CHCHD2.

Nguyen MK, McAvoy K, Liao SC, Doric Z, Lo I, Li H, Manfredi G, Nakamura K
Human molecular genetics 2021 Nov 15;

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TDP-43 and PINK1 mediate CHCHD10^S59L mutation-induced defects in Drosophila and in vitro.

Baek M, Choe YJ, Bannwarth S, Kim J, Maitra S, Dorn GW 2nd, Taylor JP, Paquis-Flucklinger V, Kim NC
Nature communications 2021 Mar 26;12(1):1924

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Loss of mitochondrial protein CHCHD10 in skeletal muscle causes neuromuscular junction impairment.

Xiao Y, Zhang J, Shu X, Bai L, Xu W, Wang A, Chen A, Tu WY, Wang J, Zhang K, Luo B, Shen C
Human molecular genetics 2020 Jul 21;29(11):1784-1796

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Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.

Peng J, Sheng AL, Xiao Q, Shen L, Ju XC, Zhang M, He ST, Wu C, Luo ZG
Journal of molecular cell biology 2019 Aug 19;11(8):636-648

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PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction.

Zhou W, Ma D, Sun AX, Tran HD, Ma DL, Singh BK, Zhou J, Zhang J, Wang D, Zhao Y, Yen PM, Goh E, Tan EK
Human molecular genetics 2019 Apr 1;28(7):1100-1116

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Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.

Straub IR, Janer A, Weraarpachai W, Zinman L, Robertson J, Rogaeva E, Shoubridge EA
Human molecular genetics 2018 Jan 1;27(1):178-189

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Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.

Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE
Nature communications 2017 Jun 6;8:15558

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse heart tissue were subjected to SDS PAGE followed by western blot with 25671-1-AP( CHCHD10 Antibody) at dilution of 1:600

Other comments

WB

Appendix

25671-1-AP.pdf

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Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 25671-1-AP( CHCHD10 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Other comments

IF

Appendix

25671-1-AP.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 25671-1-AP( CHCHD10 Antibody) at dilution of 1:50 (under 10x lens)

Other comments

IHC

Appendix

25671-1-AP.pdf

Show more