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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB3916 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RSK4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RSK4 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) RSK1, rhRSK2, or rhRSK3 is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UK32- Isotype
- IgG
- Antibody clone number
- 374521
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RSK4 by Western Blot. Western blot shows Recombinant Human Active RSK1 (Catalog # 992-KS), recombinant human RSK2, recombinant human RSK3, and recombinant human RSK4 (2 ng/lane). PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RSK4 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
