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antibody from Invitrogen Antibodies
Targeting: ATP6V1G2
ATP6G, ATP6G2, Em:AC004181.3, NG38, Vma10
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-97487 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1G2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATP6V1G2 using a ATP6V1G2 Polyclonal antibody (Product # PA5-97487) at a concentration of 12 µg/mL. Lane 1: Rat heart tissue. Lane 2: Mouse spleen tissue. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: A549 whole cell lysate. Lane 6: HT29 whole cell lysate. Lane 7: K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 14 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATP6V1G2 using a ATP6V1G2 Polyclonal antibody (Product # PA5-97487) at a concentration of 12 µg/mL. Lane 1: Rat heart tissue. Lane 2: Mouse spleen tissue. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: A549 whole cell lysate. Lane 6: HT29 whole cell lysate. Lane 7: K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 14 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP6V1G2 in A549 cells using a ATP6V1G2 polyclonal antibody (Product # PA5-97487) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATP6V1G2 in paraffin embedded human brain tissue using a ATP6V1G2 polyclonal antibody (Product # PA5-97487) at a dilution of 1:100.
