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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-24997 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA3C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model.
Importance of the putative furin recognition site 742RNRR745 for antiangiogenic Sema3C activity in vitro.
Valiulytė I, Curkūnavičiūtė R, Ribokaitė L, Kazlauskas A, Vaitkevičiūtė M, Skauminas K, Valančiūtė A
International journal of molecular sciences 2019 Nov 12;20(22)
International journal of molecular sciences 2019 Nov 12;20(22)
Importance of the putative furin recognition site 742RNRR745 for antiangiogenic Sema3C activity in vitro.
Valiulyte I, Preitakaite V, Tamasauskas A, Kazlauskas A
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2018 Oct 8;51(11):e7786
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2018 Oct 8;51(11):e7786
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SEMA3C polyclonal antibody (Product # PA5-24997) in mouse lung tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Verification of the constructed U87t-Sema3C cell line. ( A ) Microscopic images. Pictures were taken after 2 days of incubation with tetracycline. Fluorescence indicates overexpression of Sema3C protein. -Tet--no tetracycline, +Tet--1 ug/mL of tetracycline was added to the cell media. Scale bar, 100 mum. ( B ) Western blot analysis of Sema3C protein expression in U87t-Sema3C cells and cell medium. Black bar indicates Sema3C protein, approximately 85 kDa in size. Lanes 1 and 4--protein marker; lanes 2 and 5--untreated cells; lanes 3 and 6--cells treated with 1 ug/mL of tetracycline.
