Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN950423 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ankyrin Repeat Domain 34B (ANKRD34B) (C-Term), (AA 474-503) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human ANKRD34B
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 474-503
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ANKRD34B Antibody (C-term) (Cat. #AP50185PU-N) western blot analysis in 293 cell line lysates (35μg/lane).This demonstrates the ANKRD34B antibody detected the ANKRD34B protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ANKRD34B Antibody (C-term)(Cat#AP50185PU-N) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ANKRD34B Antibody (C-term) (Cat. #AP50185PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKRD34B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.