A305-831A-T

antibody from Invitrogen Antibodies

Targeting: CARMIL2 LRRC16C, RLTPR

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-831A-T

Provider

Invitrogen Antibodies

Product name

CARMIL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse CARMIL2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, MCF-7, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARMIL2 antibody A305-831A-M (lot A305-831A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CARMIL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL2 antibody (Product # A305-831A-T; lot A305-831A-T-1) used for IP at 100 µL per reaction. CARMIL2 was also immunoprecipitated by rabbit anti-CARMIL2 antibody (Product # A305-830A-T). For blotting immunoprecipitated CARMIL2, Product # A305-831A-T was used at 1:100. Detection: Chemiluminescence with an exposure time of 10 seconds.