BS3340

antibody from Bioworld Technology, Inc

Targeting: N4BP1

Immunohistochemistry

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Validation data

Product number: BS3340 - Provider product page
Provider: Bioworld Technology, Inc
Proper citation: Bioworld Technology Cat#BS3340, RRID:AB_1914328
Product name: N4BP1 (Y415) polyclonal antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide, corresponding to amino acids 391-440 of Human N4BP1.
Description: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitinÃ¿protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73Ï«, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73Ï« for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73Ï« and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100ul
Concentration: 1 mg/ml
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

N4BP1 protein structure - BS3340 shown in red.