Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-87746 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87746, RRID:AB_11034537
- Product name
- Rabbit Polyclonal Myosin VB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Myosin VB antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Editing Myosin VB Gene to Create Porcine Model of Microvillus Inclusion Disease, With Microvillus-Lined Inclusions and Alterations in Sodium Transporters.
Paxillin-dependent regulation of apical-basal polarity in mammary gland morphogenesis.
Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.
Identification of intestinal ion transport defects in microvillus inclusion disease.
Myo5b knockout mice as a model of microvillus inclusion disease.
Myosin 5b loss of function leads to defects in polarized signaling: implication for microvillus inclusion disease pathogenesis and treatment.
Engevik AC, Coutts AW, Kaji I, Rodriguez P, Ongaratto F, Saqui-Salces M, Medida RL, Meyer AR, Kolobova E, Engevik MA, Williams JA, Shub MD, Carlson DF, Melkamu T, Goldenring JR
Gastroenterology 2020 Jun;158(8):2236-2249.e9
Gastroenterology 2020 Jun;158(8):2236-2249.e9
Paxillin-dependent regulation of apical-basal polarity in mammary gland morphogenesis.
Xu W, Gulvady AC, Goreczny GJ, Olson EC, Turner CE
Development (Cambridge, England) 2019 May 1;146(9)
Development (Cambridge, England) 2019 May 1;146(9)
Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.
Forteza R, Ahsan MK, Cartón-García F, Arango D, Ameen NA, Salas PJ
Molecular biology of the cell 2019 Dec 15;30(26):3076-3089
Molecular biology of the cell 2019 Dec 15;30(26):3076-3089
Identification of intestinal ion transport defects in microvillus inclusion disease.
Kravtsov DV, Ahsan MK, Kumari V, van Ijzendoorn SC, Reyes-Mugica M, Kumar A, Gujral T, Dudeja PK, Ameen NA
American journal of physiology. Gastrointestinal and liver physiology 2016 Jul 1;311(1):G142-55
American journal of physiology. Gastrointestinal and liver physiology 2016 Jul 1;311(1):G142-55
Myo5b knockout mice as a model of microvillus inclusion disease.
Cartón-García F, Overeem AW, Nieto R, Bazzocco S, Dopeso H, Macaya I, Bilic J, Landolfi S, Hernandez-Losa J, Schwartz S Jr, Ramon y Cajal S, van Ijzendoorn SC, Arango D
Scientific reports 2015 Jul 23;5:12312
Scientific reports 2015 Jul 23;5:12312
Myosin 5b loss of function leads to defects in polarized signaling: implication for microvillus inclusion disease pathogenesis and treatment.
Kravtsov D, Mashukova A, Forteza R, Rodriguez MM, Ameen NA, Salas PJ
American journal of physiology. Gastrointestinal and liver physiology 2014 Nov 15;307(10):G992-G1001
American journal of physiology. Gastrointestinal and liver physiology 2014 Nov 15;307(10):G992-G1001
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Myosin VB Antibody [NBP1-87746] - Analysis in RT4 at 0.2 mg/mL.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues . Corresponding MYO5B RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human testis using Anti-MYO5B antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human colon, kidney, lymph node and testis using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human lymph node using Anti-MYO5B antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human kidney using Anti-MYO5B antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human colon using Anti-MYO5B antibody.