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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- PA5-57948 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MENT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SSTRFIANSQ EPEIRLTSSL PRSPGRSTED LPGSQATLSQ WSTPGSTPSR WPSPSPTAMP SPEDLRLVLM PWGPWHCHCK SGTMS
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MENT in control (vector only transfected HEK293T lysate) and C1orf56 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MENT Polyclonal Antibody (Product # PA5-57948).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MENT in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a MENT Polyclonal Antibody (Product # PA5-57948).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human liver using MENT Polyclonal Antibody (Product # PA5-57948).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human lymph node using MENT Polyclonal Antibody (Product # PA5-57948).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human colon using MENT Polyclonal Antibody (Product # PA5-57948).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human testis and prostate tissues using MENT Polyclonal Antibody (Product # PA5-57948). Corresponding C1orf56 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human colon, liver, lymph node and testis using MENT Polyclonal Antibody (Product # PA5-57948) (A) shows similar protein distribution across tissues to an independent MENT Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human testis using MENT Polyclonal Antibody (Product # PA5-57948) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MENT in human prostate using MENT Polyclonal Antibody (Product # PA5-57948) shows low expression as expected.
