MAB7676

antibody from Novus Biologicals

Targeting: FRMPD4 KIAA0316, PDZD10, PDZK10

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7676

Provider

Novus Biologicals

Product name

Mouse Monoclonal Preso/FRMPD4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Preso/FRMPD4 in direct ELISAs and human and mouse Preso/FRMPD4 in Western blots.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Preso/FRMPD4 by Simple Western^TM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/mL. A specific band was detected for Preso/FRMPD4 at approximately 170 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Preso/FRMPD4 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Preso/FRMPD4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.