Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-63946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPACA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GVKDCVFCEL TDSMQCPGTY M
- Concentration
- 0.05 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPACA4 in control (vector only transfected HEK293T lysate) and SPACA4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPACA4 Polyclonal Antibody (Product # PA5-63946).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human colon using SPACA4 Polyclonal Antibody (Product # PA5-63946).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human lymph node using SPACA4 Polyclonal Antibody (Product # PA5-63946).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human cerebral cortex using SPACA4 Polyclonal Antibody (Product # PA5-63946).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human testis and endometrium tissues using SPACA4 Polyclonal Antibody (Product # PA5-63946). Corresponding SPACA4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human cerebral cortex, colon, lymph node and testis using SPACA4 Polyclonal Antibody (Product # PA5-63946) (A) shows similar protein distribution across tissues to an independent SPACA4 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human testis using SPACA4 Polyclonal Antibody (Product # PA5-63946) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA4 in human endometrium using SPACA4 Polyclonal Antibody (Product # PA5-63946) shows low expression as expected.
