PAB23383

antibody from Abnova Corporation

Targeting: PATL1 FLJ36874, Pat1b

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23383

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23383, RRID:AB_11126524

Product name

PATL1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PATL1.

Antigen sequence

QHRRLLHQRQQQNRSQHRNLNGAGDRGSHRSSHQD
HLRKDPYANLMLQREKDWVSKIQMMQLQSTDPYLD
DFYYQNYFEKLEKLSAAEEIQGDGPKKE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with PATL1 polyclonal antibody (Cat # PAB23383).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with PATL1 polyclonal antibody (Cat # PAB23383) at 1-4 ug/mL dilution shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with PATL1 polyclonal antibody (Cat # PAB23383) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)