PAB28000

antibody from Abnova Corporation

Targeting: ACOD1 CAD, IRG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28000

Provider

Abnova Corporation

Product name

IRG1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant IRG1

Antigen sequence

FPAHLSTHWVADAAASVRKHLVAERALLPTDYIKR
IVLRIPNVQYVNRPFPVSEHEARHSFQYVACAMLL
DGGITVPSFHECQINRPQVRELLSKVELEY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4 Lane 2: U-251 MG Lane 3: Human Plasma Lane 4: Liver Lane 5: Tonsil with IRG1 polyclonal antibody ( Cat # PAB28000 ) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with IRG1 polyclonal antibody ( Cat # PAB28000 ) at 1-4 ug/ml dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with IRG1 polyclonal antibody ( Cat # PAB28000 ) shows strong nuclear positivity in glandular cells at 1:200 - 1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)