HPA037469

antibody from Atlas Antibodies

Targeting: DNAH5 CILD3, Dnahc5, HL1, KTGNR, PCD

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA037469

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA037469, RRID:AB_10672791

Product name

Anti-DNAH5

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NFDTLTSSINARANALLLTTVTRKKKETEMLGEEA
RELLSHFNHQNMDALLKVTRNTLEAIRKRIHSSHT
INFRDSNSASNMKQNSLP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and liver tissues using HPA037469 antibody. Corresponding DNAH5 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, fallopian tube, liver and nasopharynx using Anti-DNAH5 antibody HPA037469 (A) shows similar protein distribution across tissues to independent antibody HPA037470 (B).

Antibody #2 product nr

HPA037470

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-DNAH5 antibody HPA037469.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-DNAH5 antibody HPA037469.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human nasopharynx using Anti-DNAH5 antibody HPA037469.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows no positivity as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Fallopian tube shows strong positivity in cilia in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows no positivity in neurons as expected.

Sample type

HUMAN