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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-68424 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAT8L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with Mouse and Rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- -20°C
Submitted references The cAMP Inducers Modify N-Acetylaspartate Metabolism in Wistar Rat Brain.
Kowalski R, Pikul P, Lewandowski K, Sakowicz-Burkiewicz M, Pawełczyk T, Zyśk M
Antioxidants (Basel, Switzerland) 2021 Sep 1;10(9)
Antioxidants (Basel, Switzerland) 2021 Sep 1;10(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAT8L in lane 1-2: 293T and A172 cell lysates (40µg lysate/lane). Samples were loaded onto a 12% SDS-PAGE gel and probed using a NAT8L Polyclonal Antibody (Product # PA5-68424) at a 1:250 dilution, followed by incubation with a Goat anti rabbit IgG secondary antibody at a 1:8000 dilution, Exposure time: 45 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Impact of the maturation processes on the NAT8L. NAT8L tagged by PA5-68424 or MBS9216916 antibodies ( A1 ) NAT8L predicted structure (PDB ID: AF-Q8N9F0-F1) with marked epitopes. Illustrations edited as per author's permission statement, available under a CC-BY-4.0 License; ( A2 ) WB membrane images of brain homogenates; ( B ) representative WB membrane images of brain homogenates or skin tissue (negative control), NAT8L tagged by PA5-68424 antibody, ( C ) representative WB membrane images of NSC ( C1 ) and brain homogenates ( C2 ), NAT8L tagged by PA5-68424, beta-actin was used as a reference protein; ( D ) NAT8L protein level calculated from WB membrane, band: 36 kDa; ( E ) NAT8L activity; ( G ) Nat8l mRNA level (RT-qPCR); ( H ) fluorescence microscopy of primary neurons (PR) and neural stem cells (NSC), white scale bar represents 20 um, images are representative for 3 independent experiments. Results are showed as medians with interquartile ranges from 4-15 observations per group. Significantly different from the control: * ( p -value < 0.05), ** ( p -value < 0.01), *** ( p -value < 0.001). Abbreviations: NSC: neural stem cells; PR: primary neurons.
