PAB28249

antibody from Abnova Corporation

Targeting: HS3ST5 3-OST-5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28249

Provider

Abnova Corporation

Product name

HS3ST5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HS3ST5.

Antigen sequence

DRLQPICPIEGRLGGARTQAEFPLRALQFKRGLLH
EFRKGNASKEQVRLHDLVQQLPKAIIIGVRKGGTR
ALLEMLNLHPAVVKASQEIHF


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with HS3ST5 polyclonal antibody (Cat # PAB28249).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with HS3ST5 polyclonal antibody (Cat # PAB28249) at 1-4 ug/ml shows positivity in focal adhesions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with HS3ST5 polyclonal antibody (Cat # PAB28249) shows strong cytoplasmic positivity in Purkinje cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)