GTX22810

antibody from GeneTex

Targeting: RAB9A RAB9

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX22810

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22810, RRID:AB_384866

Product name

Rab9 antibody [Mab9]

Antibody type

Monoclonal

Reactivity

Human, Rat, Bovine, Canine, Hamster, Simian

Host

Mouse

References

Submitted references

Rotaviruses reach late endosomes and require the cation-dependent mannose-6-phosphate receptor and the activity of cathepsin proteases to enter the cell.

Díaz-Salinas MA, Silva-Ayala D, López S, Arias CF
Journal of virology 2014 Apr;88(8):4389-402

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Rab9 in A2058 Cells. Rab9 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Rab9 antibody [Mab9] at a dilution of 1:100 over night at 4 ¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry was performed on cancer biopsies of deparaffinized human prostate carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without Rab9 antibody [Mab9] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.