AP7614a

antibody from Abgent

Targeting: EPHA10 FLJ16103, FLJ33655

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7614a

Provider

Abgent

Proper citation

Abgent Cat#AP7614a, RRID:AB_2098937

Product name

EPHA10 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of EPHA10 Antibody (N-term) (Cat.#AP7614a) in K562 cell line lysates (35ug/lane). EPHA10 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of EPHA10 (arrow) using rabbit polyclonal EPHA10 Antibody (N-term) (Cat. #AP7614a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml)

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human testis tissue reacted with EPHA10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50